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Interspecies cell fusion between mouse embryonic stem cell and porcine pluripotent cell
Author(s) -
Bou Gerelchimeg,
Guo Jia,
Fang Yuan,
Li Xuechun,
Wei Renyue,
Li Yan,
Liu Zhonghua
Publication year - 2021
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13952
Subject(s) - induced pluripotent stem cell , reprogramming , embryonic stem cell , biology , somatic cell , microbiology and biotechnology , cell potency , stem cell , cell fusion , induced stem cells , embryoid body , cell , genetics , gene
In the area of stem cell research, fusion of somatic cells into pluripotent cells such as mouse embryonic stem (ES) cells induces reprogramming of the somatic nucleus and can be used to study the effect of trans‐acting factors from the pluripotent cell on the pluripotent state of somatic nucleus. As many other groups, we previously established a porcine pluripotent cell line at a low potential. Therefore, here, we performed experiments to investigate if the fusion with mouse ES cell could improve the pluripotent state of porcine pluripotent cell. Our data showed that resultant mouse–porcine interspecies fused cells are AP positive, and could be passaged up to 20 passages. Different degrees of increases in expression of porcine pluripotent genes proved that pig‐origin gene network can be programmed by mouse ES. Further differentiation study also confirmed these fused cells’ potential to form three germ layers. However, unexpectedly, we found that chromosome loss and aberrant (especially in porcine chromosomes) is severe after the cell fusion, implying that interspecies cell fusion may be not suitable to study porcine pluripotency without additional supportive conditions for genome stabilization.