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Evaluation of sperm morphometry of rabbits ( Oryctolagus cuniculus f. domesticus )
Author(s) -
Szeleszczuk Olga,
Andraszek Katarzyna,
Banaszewska Dorota,
Niedbała Piotr,
KuchtaGładysz Marta,
Grzesiakowska Anna
Publication year - 2021
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13876
Subject(s) - sperm , spermatozoon , staining , biology , silver nitrate , semen , anatomy , andrology , sperm motility , silver stain , chemistry , botany , microbiology and biotechnology , medicine , genetics , nuclear chemistry
Contents Sperm morphology and morphometry are considered parameters in fertility diagnosis. They are especially important in the case of species for which there is no standard with respect to morphometric sperm parameters. It is then crucial to apply the staining technique that has the least influence on the sperm structure and provides the most detailed image, so as to enable measurements. The aim of the research was to assess the morphometric parameters of rabbit sperm using silver nitrate staining. The staining process revealed a detailed image of the spermatozoon head and tail, thus enabling precise measurements. From these basic morphometric parameters, four additional shape indices characterizing the sperm head were calculated: ellipticity, elongation, roughness and regularity. These parameters more precisely characterize the shape of the sperm head. Silver nitrate staining can be used as an independent technique in assessment of sperm structure or to supplement routine diagnostics.