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Melatonin can improve viability and functional integrity of cooled and frozen/thawed rabbit spermatozoa
Author(s) -
Fadl Aya M.,
Ghallab Abdel Raouf M.,
AbouAhmed Mostafa M.,
Moawad Adel R.
Publication year - 2021
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13853
Subject(s) - extender , semen , semen extender , melatonin , andrology , sperm , acrosome , semen quality , cryopreservation , semen cryopreservation , biology , insemination , artificial insemination , sperm motility , chemistry , zoology , endocrinology , medicine , embryo , pregnancy , microbiology and biotechnology , genetics , organic chemistry , polyurethane
Abstract Melatonin is known to protect sperm against freezing‐inflicted damage in different domestic species. The aim of the study was to evaluate the effect of supplementation of semen extender with melatonin on the quality and DNA integrity of cooled and frozen/thawed rabbit spermatozoa. We also investigated whether the addition of melatonin to the semen extender could improve the fertility of rabbit does artificially inseminated with frozen/thawed semen. Semen samples collected from eight rabbit bucks were pooled and then diluted in INRA‐82 supplemented either with (0.5, 1.0 or 1.5 mM) or without (0.0 mM) melatonin. Diluted semen was cooled at 5°C for 24 hr. For cryopreservation and based on the first experiment's best result, semen samples were diluted in INRA‐82 in the presence or absence of 1.0 mM melatonin and then frozen in 0.25 ml straws. Following cooling or thawing, sperm quality and DNA integrity were evaluated. Furthermore, the fertility of frozen/thawed semen was investigated after artificial insemination. Supplementation of semen extender with 1.0 mM melatonin improved ( p < .05) motility, viability, membrane and acrosome integrities in cooled semen compared with other groups. Sperm quality and DNA integrity were higher ( p < .05) in frozen/thawed semen diluted in 1.0 mM melatonin‐supplemented extender than in the control group. Conception and birth rates were higher in does inseminated with 1.0 mM melatonin treated semen compared with the controls. In conclusion, supplementation of semen extender with 1.0 mM melatonin improved the quality of cooled and frozen/thawed rabbit spermatozoa. Melatonin can preserve DNA integrity and enhance the fertility of frozen/thawed rabbit spermatozoa.