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Ascorbic acid ameliorates dysregulated folliculogenesis induced by mono‐(2‐ethylhexyl)phthalate in neonatal mouse ovaries via reducing ovarian oxidative stress
Author(s) -
Liu Chang,
Shui Shike,
Yao Yangcheng,
Sui Cong,
Zhang Hanwang
Publication year - 2020
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13790
Subject(s) - folliculogenesis , oxidative stress , medicine , malondialdehyde , ovarian follicle , endocrinology , phthalate , ascorbic acid , ovary , reactive oxygen species , follicle , andrology , chemistry , biology , biochemistry , embryo , embryogenesis , food science , microbiology and biotechnology , organic chemistry
Phthalates, including di‐(2‐ethylhexyl)phthalate (DEHP), are common industrial chemicals in the environment. Recent evidence indicates that DEHP and its active metabolite mono‐(2‐ethylhexyl)phthalate (MEHP) negatively modulate reproductive functions and induce reactive oxygen species. Ascorbic acid (AA) is a dietary requirement for primates, and it acts as a potent free radical scavenger to protect tissues against oxidative stress. In this study, to investigate the toxic effects of MEHP on the follicle development and the beneficial role of AA, neonatal mouse ovaries were treated with different concentrations of MEHP with or without AA for 6 days. Then, the follicle constitution and oxidative status were compared in different groups. Results showed MEHP accelerated primordial follicle recruitment by increasing the percentage of primary and secondary follicles and decreasing the percentage of primordial follicles in the ovaries. Moreover, MEHP‐induced ovarian oxidative stress by significantly increasing malondialdehyde (MDA) concentration and the expression of GSS and SOD1. When ovaries were co‐administrated with MEHP and AA, follicle constitution was normalized, and the oxidative status was significantly decreased. These results suggested that AA ameliorated MEHP‐induced ovarian oxidative stress and follicular dysregulation, which attested the clinical significance of AA for ovary protection in the case of MEHP exposure.