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TGIF1 and SF1 polymorphisms are associated with litter size in Small Tail Han sheep
Author(s) -
Zhang Zhuangbiao,
He Xiaoyun,
Liu Qiuyue,
Tang Jishun,
Di Ran,
Chu Mingxing
Publication year - 2020
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13753
Subject(s) - biology , litter , genetics , locus (genetics) , genotype , gene , ecology
TGF‐β induced factor homeobox 1 ( TGIF1 ) and splicing factor 1 ( SF1 ) are important for mammalian reproduction; however, the effects of these genes on litter size in sheep remain unexplored. In this study, we genotyped 768 ewes from seven sheep breeds at two loci: g.37871539C>T, a synonymous mutation of TGIF1 ; and g.42314637T>C, a 3′UTR variant of SF1 . Our analysis of polymorphism revealed only two genotypes at locus g.37871539C>T in TGIF1 , with most sheep populations being moderately polymorphic (0.25 < PIC < 0.5) at this site. In contrast, most breeds exhibited low polymorphism (PIC ≤0.25) at the SF1 locus g.42314637T>C. The association analysis revealed that a synonymous mutation at g.37871539C>T in TGIF1 was highly associated with litter size in Small Tail Han sheep, in which it causes a significant decrease in litter size. Conversely, while the SF1 3′UTR variant g.42314637T>C was also highly associated with litter size in sheep, it causes a significant increase in the number of litter size. Combined, these data provide valuable information regarding candidate genetic markers for sheep breeding programs.