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The relationship between acrosome reaction and polyunsaturated fatty acid composition in boar sperm
Author(s) -
Lee SangHee,
Kim YuJin,
Kang Byeong Ho,
Yun Yong Sik,
Park ChoonKeun
Publication year - 2020
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13661
Subject(s) - acrosome reaction , acrosome , sperm , polyunsaturated fatty acid , docosapentaenoic acid , chemistry , andrology , docosahexaenoic acid , boar , fatty acid , biochemistry , medicine
This study investigated the relationship between acrosome reactions and fatty acid composition with respect to fertility in boar sperm. The acrosome reaction was induced more than 85% by 60 mM methyl‐beta‐cyclodextrin (MBCD), and plasma membrane integrity was significantly reduced dependent on the MBCD level in boar sperm ( p < .05). The acrosome‐reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non‐acrosome reaction group ( p < .0001). In addition, the PUFAs, C22:5n‐6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n‐3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly ( p < .0001) decreased in acrosome‐reacted sperm relative to non‐acrosome‐reacted sperm. Moreover, acrosome reaction was positively correlated with SFAs, whereas negatively correlated with PUFAs, of the PUFAs, the DPA ( p = .0005) and DHA ( p = <.0001) were negatively correlated with the acrosome reaction. Therefore, these results suggest that the PUFAs composition of sperm is closely involved in acrosome reaction in pigs.