Premium
The role of neurokinin A and its receptor in the regulation of prolactin secretion by the anterior pituitary of cyclic pigs
Author(s) -
Czelejewska Wioleta,
Zmijewska Agata,
Dziekonski Mariusz,
Okrasa Stanislaw
Publication year - 2020
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13659
Subject(s) - prolactin , medicine , endocrinology , neurokinin b , estrous cycle , secretion , receptor , prolactin receptor , biology , anterior pituitary , neurokinin a , prolactin cell , pituitary gland , hypothalamus , chemistry , hormone , substance p , neuropeptide
In pigs, plasma prolactin concentration markedly changes during the oestrous cycle and the regulation of its secretion is very complex. The contribution of neurokinins in this process has not been sufficiently delineated. The aim of the study was to examine the effects of neurokinin A (NKA) on prolactin synthesis and secretion in cyclic gilts. The expression of NKA precursor ( Ppta ) and receptor ( Tacr2 ) genes as well as NKA and TACR proteins content in the porcine pituitaries (days 2–3, 9–10, 12–13, 15–16 and 19–20 of the cycle) was determined. Furthermore, the in vitro influence of NKA on the expression of prolactin ( Prl ), dopamine receptor ( D2r ), TRH receptor ( Trhr ) genes and prolactin secretion by the porcine pituitary cells (days 9–10, 15–16 and 19–20 of the cycle) was assessed. The expression of Ppta and Tacr2 as well as NKA and TACR proteins in the pituitary tissue has been changing throughout the oestrous cycle. NKA affected in vitro the expression of studied genes and prolactin secretion depending on the stage of the cycle, dose of NKA and/or duration of the cell incubation. Altogether, the study indicates that NKA is engaged in the modulation of prolactin secretion in the pig during the oestrous cycle.