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Studies of gel with immobilized semen by intrauterine endoscopy post‐artificial insemination
Author(s) -
Berg Halldor Felde,
Spång Heidi Cecilie Larsen,
Heringstad Bjørg,
Ropstad Erik,
AlmKristiansen Anne Hege,
Kommisrud Elisabeth
Publication year - 2020
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13630
Subject(s) - semen , incubation , insemination , andrology , artificial insemination , uterus , in vivo , sperm , ex vivo , biology , sperm washing , motility , incubation period , in utero , medicine , pregnancy , endocrinology , fetus , biochemistry , microbiology and biotechnology , genetics
An extended lifespan of spermatozoa following artificial insemination (AI) can make the timing of insemination less critical, as previously demonstrated with immobilized spermatozoa that are gradually released from an alginate gel. The purpose was to examine the in vivo dissolution of SpermVital (SV) alginate gel over time by endoscopy and secondly to assess spermatozoa quality after incubation of the gel. In vivo endoscopy showed SV gel in the uterus 3, 6, 20 and 24 hr after AI, demonstrating the potential release of spermatozoa to the uterus during this period. In utero ex vivo incubation of the semen demonstrated that high motility and viability of sperm cells was sustained following overnight incubation.