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Ovum pick‐up and in vitro maturation in spotted paca ( Cuniculus paca —Linnaeus, 1766)
Author(s) -
Barros Felipe Farias Pereira da Câmara,
Teixeira Pedro Paulo Maia,
PadilhaNakaghi Luciana Cristina,
Uscategui Ricardo Andres Ramirez,
Lima Marina Ragagnin,
Santos Victor José Correia,
Rossy Kayan da Cunha,
Borges Luísa Pucci Bueno,
Machado Marcia Rita Fernandes,
Vicente Wilter Ricardo Russiano
Publication year - 2020
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13627
Subject(s) - follicular phase , saline , oocyte , medicine , endocrinology , andrology , biology , stimulation , ovary , physiological saline , embryo , microbiology and biotechnology
Abstract We tested FSHp, eCG and FSHp + eCG to establish ovum pick‐up (OPU) and in vitro maturation method in spotted paca. Eight healthy adult females were subjected to each of four treatments to stimulate ovarian follicular growth. All females were subjected to a hormonal protocol using a single dose of 45 mg of injectable progesterone and single intramuscular injection of 0.075 mg d‐cloprostenol on day 6. Ovarian stimulation was carried out as follows: in Group TFE (FSHp and eCG), animals were treated with a single dose of 80 mg of FSHp and 200 IU of eCG intramuscularly on day 6 after the application of progesterone; in Group TF (FSHp), they were treated with a single dose of 80 mg of FSHp intramuscularly on day 6 after application of progesterone; in Group treatment eCG, they were treated with 200 IU of eCG intramuscularly on day 6 after application of progesterone; and in Group TC (saline solution), 1 ml of saline solution was administered to control does. The OPU was performed between 22 and 26 hr after gonadotropin treatments. All recovered oocytes were placed into maturation media and incubated for 24 hr. There were no differences among the mean number of observed follicles, aspirated follicles and oocytes recovered per treatment. Oocyte maturation rates did not differ among groups, except, TF and treatment eCG oocytes had greater maturation rates than TC oocytes. In this study, gonadotropin administration failed to superovulate treated does and increase oocyte retrieval efficiency. Despite the feasibility of the procedure, further studies are needed to develop and refine hormonal protocols for oocyte recovery and in vitro maturation in this species.