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Antibiotic resistance in microorganisms isolated in a bull semen stud
Author(s) -
Goularte Karina L.,
Voloski Flávia L. S.,
Redú Josiara F. M.,
Ferreira Carlos E. R.,
Vieira Arnaldo D.,
Duval Eduarda H.,
Mondadori Rafael G.,
Lucia Thomaz
Publication year - 2020
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13621
Subject(s) - antibiotics , microorganism , penicillin , lincomycin , tylosin , biology , microbiology and biotechnology , semen , antibiotic resistance , bacteria , veterinary medicine , medicine , genetics
Many microorganisms from various sources may be present in ejaculates of bulls. This study identified and isolated bacteria from bull sperm samples in a commercial stud and evaluated their resistance to antibiotics. The number of colony‐forming units was determined in semen samples collected at distinct steps during freezing and thawing. The minimum inhibitory concentration and the minimum bactericidal concentration were determined for four antibiotics commonly used in commercial studs. A total of 135 microorganisms from 25 genera were isolated. After a sensitivity test, all evaluated microorganisms ( n = 55) were resistant to penicillin and most of them were resistant to tylosin and lincomycin ( n = 54). Resistance to all tested antibiotics was observed in 22% of all isolates, whereas only 3.9% of the isolates were inhibited by the tested antibiotics at the concentrations recommended by the international legislation. As the isolated microorganisms presented high resistance to frequently used antibiotics, sensitivity tests should be periodically conducted in commercial bull semen studs to prevent the use of contaminated semen in artificial insemination.