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Comparative evaluation of DNA integrity using sperm chromatin structure assay and Sperm‐Ovis‐Halomax during in vitro capacitation of cryopreserved ram spermatozoa
Author(s) -
PerisFrau Patricia,
ÁlvarezRodríguez Manuel,
MartínMaestro Alicia,
IniestaCuerda María,
SánchezAjofrín Irene,
Garde José Julián,
RodriguezMartinez Heriberto,
Soler Ana Josefa
Publication year - 2019
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13519
Subject(s) - sperm , dna fragmentation , capacitation , andrology , ovis , biology , cryopreservation , semen , incubation , chromatin , dna , anatomy , embryo , genetics , apoptosis , biochemistry , medicine , ecology , programmed cell death
This study aimed to compare the ability of sperm chromatin structure assay ( SCSA ® ) and Sperm‐Ovis‐Halomax ® to detect DNA fragmentation in frozen‐thawed ram spermatozoa incubated under capacitating conditions in synthetic oviductal fluid ( SOF ) supplemented with oestrous sheep serum ( SOF ‐ ESS ) at multiple time points (0–240 min). Incubation in SOF ‐ ESS had no significant effects on SCSA ® parameters while the percentage of spermatozoa with fragmented DNA measured by Sperm‐Ovis‐Halomax ® increased after 180 min of incubation. In addition, no correlation or agreement was found between the techniques, suggesting that SCSA ® and Sperm‐Ovis‐Halomax ® may quantify different types of DNA damage in ram spermatozoa under these experimental conditions.