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Identification of the JY‐1 in sika deer and its effects on granulosa cells cultured in vitro
Author(s) -
Chen Xiumin,
Xing Mingjie,
Yang Yifeng,
Chen Min,
Chang Tong,
Xu Baozeng,
Wei Haijun
Publication year - 2019
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13471
Subject(s) - biology , gene knockdown , in vitro , andrology , oocyte , microbiology and biotechnology , apoptosis , embryo , genetics , medicine
The oocyte‐specific protein JY‐1 was reported as an important regulator of both function of ovarian granulosa cells and early embryogenesis in cattle. Here, we found that the transcripts of JY‐1 were also present in sika deer granulosa cells (GCs) through in situ hybridization and qRT‐PCR. The complete sika deer JY‐1 coding sequence was identified, which had three exons separated by two introns. It was detected that JY‐1 knockdown caused apoptosis and abnormal cell cycle progression in GCs of sika deer cultured in vitro. Taken together, these data suggest that JY‐1 is involved in the regulation of proliferation in sika deer ovarian GCs.