Premium
Genetic Polymorphisms in LTF/Eco RI and TLR4/Alu I loci as candidates for milk and reproductive performance assessment in Holstein cattle
Author(s) -
ElDomany Wael B.,
Radwan Hend A.,
Ateya Ahmed I.,
Ramadan Hazem H.,
Marghani Basma H.,
Nasr Sherif M.
Publication year - 2019
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13408
Subject(s) - biology , genotype , ice calving , lactation , zoology , allele , genetics , gene , pregnancy
Contents The aim of this study was to explore the genetic polymorphisms in LTF/Eco RI and TLR4/Alu I loci and their association with milk and reproductive performance in Holstein cattle. A randomly selected 800 Holstein dairy cows from two dairy farms (400 animals each) in Egypt were used. Based on the two farm records, association between LTF/Eco RI genotypes and milk performance traits (order of lactation, daily milk yield, days in milk, corrected milk at 305 day and dry period) was carried out. Meanwhile, exploring of TLR4/Alu I genotypes effect was done on data for reproductive performance (age at first freshening, calving interval, number of services per conception, ovarian rebound and days open). DNA was extracted from blood samples collected from Holstein dairy cows of the both farms and restriction analysis of 301‐bp PCR products of LTF gene revealed two genotypes: AA genotype (301 bp) and AB genotype (301, 201 and 100 bp). Meanwhile, restriction analysis of 382‐bp PCR products of TLR4 gene digested with Alu I yielded two alleles (A and B) and three genotypes (AA, AB and BB). The A allele was indicated by two bands at 300 and 82 bp, and the B allele resulted in three fragments of 160, 140 and 82 bp. There was a significant association ( p ≤ 0.05) between LTF genotypes and milk performance traits except for days in milk. The TLR4 genotypes had significant effects ( p ≤ 0.05) on age at first freshening, calving interval, number of services per conception, ovarian rebound and days open. Ordinal logistic regression statistical model also revealed that it is possible to calculate high reproductive performance traits and to predict favourable dairy cows based on LTF and TLR4 genotypes. This research reveals the effectiveness of LTF/Eco RI and TLR4/Alu I loci as candidates for reproductive performance assessment in Holstein cattle.