Premium
Relationship between motile sperm subpopulations identified in frozen‐thawed dog semen samples and their ability to bind to the zona pellucida of canine oocytes
Author(s) -
Peña Ana I.,
Adán Sheila,
Quintela Luis A.,
Becerra Juan J.,
Herradón Pedro G.
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13349
Subject(s) - zona pellucida , sperm , semen , andrology , biology , oocyte , sperm motility , anatomy , embryo , microbiology and biotechnology , medicine
Contents Studies performed on ejaculates from several species have identified discrete subpopulations of motile sperm. In dogs, motile sperm subpopulations have also been described in fresh and frozen‐thawed semen. The subpopulation of the most rapid and progressively motile sperm has been suggested to be the most likely source of fertilizing spermatozoa. However, the significance of subpopulation differences among dogs and ejaculates relative to fertility is not known. The aim of this study was to investigate the relationship between the relative proportion of motile sperm subpopulations in frozen‐thawed dog semen samples and their ability to bind to the zona pellucida of canine oocytes. Multiple linear regression analysis indicated that the subpopulation of the most rapid and progressively motile sperm was significantly and positively correlated with zona pellucida‐binding assays (ZBA) outcomes: each 10% increase in this subpopulation was associated with an increase of 1.5 sperm bound per oocyte. Subpopulations of hyperactivated‐like or locally motile sperm were negatively correlated with the ZBA results. It was concluded that subpopulation differences among frozen‐thawed dog semen samples determined differences in the number of sperm bound to the ZP of canine oocytes.