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A polymorphism in the transcriptional regulatory region strongly influences ovine FSHR mRNA decay
Author(s) -
Wang Dedi,
Du Xing,
Li Yinxia,
Li Qifa
Publication year - 2019
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13316
Subject(s) - three prime untranslated region , follicle stimulating hormone receptor , untranslated region , messenger rna , biology , gene , transcription (linguistics) , microbiology and biotechnology , five prime untranslated region , transcriptional regulation , receptor , genetics , hormone receptor , gene expression , linguistics , philosophy , cancer , breast cancer
Contents Follicle‐stimulating hormone receptor (FSHR) is an important G protein‐coupled receptor, which is required for steroidogenesis, follicular development and female infertility. Here, we report a novel polymorphism in the 3′‐UTR that strongly influences ovine FSHR mRNA decay. The partial 3′‐UTR sequence of Hu sheep FSHR gene was isolated and characterized, and a polymorphism (c.2327A>G) was identified. Luciferase assay and qRT‐PCR showed that c.2327A>G polymorphism in the 3′‐UTR exerts a strong regulatory role in FSHR transcription. This regulatory role is achieved by affecting FSHR mRNA decay. Furthermore, the c.2327A>G mutation in the 3′‐UTR influences ARE (AU‐rich element, a cis‐acting element promoting mRNA decay)‐mediated mRNA decay of Hu sheep FSHR gene. Together, our study identified a novel polymorphism and elucidated a new mechanism underlying transcriptional regulation of FSHR in mammals.