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Extracellular vesicles derived from endometrial human mesenchymal stem cells improve IVF outcome in an aged murine model
Author(s) -
Marinaro Federica,
Pericuesta Eva,
SánchezMargallo Francisco Miguel,
Casado Javier G.,
Álvarez Verónica,
Matilla Elvira,
Hernández Nuria,
Blázquez Rebeca,
GonzálezFernández Lauro,
GutiérrezAdán Alfonso,
MacíasGarcía Beatriz
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13314
Subject(s) - andrology , blastocyst , mesenchymal stem cell , glutathione peroxidase , gpx1 , superoxide dismutase , biology , embryo , reactive oxygen species , chemistry , endocrinology , embryogenesis , oxidative stress , microbiology and biotechnology , medicine
Advanced age reduces the success of in vitro fertilization ( IVF ) being this effect partly mediated by an overproduction of reactive oxygen species ( ROS ) that trigger apoptosis. It has been demonstrated that extracellular vesicles derived from endometrial mesenchymal stem cells ( EV ‐end MSC s) exert an antioxidant effect and can be used as IVF coadjutants. In this work, end MSC s were isolated from human menstrual blood ( n = 4) and characterized according to multipotentiality and surface marker expression prior EV ‐end MSC s isolation. Oocytes were obtained from 21 B6D2 mice (24 weeks) and coincubated with sperm from young males (8–12 weeks). Presumptive zygotes were incubated in the presence of 0, 10, 20, 40 or 80 μg/ml of EV ‐end MSC s in KSOM medium. Blastocyst yield was evaluated, and 25 blastocysts per group were used for qPCR . Blastocyst rate was 29.4% in control; 45.2% for 10 μg/ml, 62.9% for 20 μg/ml, 55.5% for 40 μg/ml and 53.8% in the 80 μg/ml ( n = 124–130 oocytes) being all the increases significantly different when compared against control ( p < 0.05). The 20–80 μg/ml treatments decreased the expression of glutathione peroxidase ( Gpx1 ), and the 10–40 μg/ml treatments reduced the expression of superoxide dismutase ( Sod1 ; p < 0.05) compared to control; Bax mRNA expression did not vary. Our results suggest that the increased developmental competence of the embryos could be partly mediated by the EV ‐end MSC s’ ROS scavenger activity.