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Effect of different oxidative stress degrees generated by hydrogen peroxide on motility and DNA fragmentation of zebrafish ( Danio rerio ) spermatozoa
Author(s) -
Sadeghi Sara,
Pertusa José,
Yaniz Jesús L.,
Nuñez Javier,
Soler Carles,
Silvestre Miguel Angel
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13296
Subject(s) - motility , oxidative stress , dna fragmentation , sperm motility , sperm , andrology , semen , lipid peroxidation , reactive oxygen species , antioxidant , fragmentation (computing) , chemistry , danio , biology , zebrafish , biochemistry , microbiology and biotechnology , apoptosis , programmed cell death , medicine , ecology , gene
Contents An increase in reactive oxygen species (ROS) or decrease in antioxidant barriers can provoke lipid peroxidation of the membranes or DNA damage of the spermatozoa. The aim of this work is to study the effect of the different degrees of oxidative stress generated by H 2 O 2 incubation on total motility, kinetics, and DNA fragmentation of zebrafish ( Danio rerio) spermatozoa. For this process, experimental groups were incubated in 50 µM (Low; L) and 200 µM (High; H) H 2 O 2 , respectively, for 20 min at 4ºC. Sperm motility parameters were obtained with a computer‐assisted sperm analysis (CASA) system. Sperm DNA fragmentation (SDF) was assessed using the sperm chromatin dispersion test. Both low and high H 2 O 2 concentration groups showed lower motility than control groups. Progressive motility of spermatozoa incubated in the H group dropped rapidly in comparison with other groups. Regarding SDF, the control and L groups had significantly lower values than the H group (25.0% and 31.6% vs. 48.1% fragmented sperm for C, L, and H groups, respectively; p < 0.05). Sperm motility, mostly progressive motility, decreased as H 2 O 2 concentration increased, mainly when time after sperm activation increased. SDF increased as the H 2 O 2 concentration increased. However, measurements of the halo area did not agree with the subjective SDF rate.