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Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern
Author(s) -
Gomes Elizabete T.,
Costa Joana Amélia S.,
Silva Diogo Manoel F.,
Al Shebli Wasim,
Azevedo Marina L.,
Monteiro Jr Pedro L. J.,
Araújo Silva Robespierre A. J.,
Santos Filho Antônio S.,
Guerra Maria Madalena P.,
Bartolomeu Claudio Coutinho,
Wischral Aurea,
Batista André M.
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13251
Subject(s) - adiponectin , ampk , medicine , endocrinology , mapk/erk pathway , oocyte , protein kinase a , adipokine , adipose tissue , adiponectin receptor 1 , signal transduction , biology , chemistry , microbiology and biotechnology , kinase , embryo , leptin , obesity , insulin resistance
Contents Recent studies have shown that adiponectin, an adipokine predominantly produced by adipose tissue, regulates several reproductive processes. However, the mechanisms of action of adiponectin on the maturation of goat oocytes remain to be determined. The aim of this study was to investigate whether (a) adiponectin influences the meiotic maturation of goat oocytes; (b) MAPK MEK 1/2 mediates the effects of adiponectin; and 3) adiponectin differentially affects mRNA relative abundance of genes relevant for adiponectin signal transduction in goat oocytes. The addition of adiponectin (5 μg/ml) during the maturation of goat oocytes resulted in a higher percentage of successful nuclear maturation compared to those of the group without adiponectin ( p < 0.05). Adiponectin‐stimulated nuclear oocyte maturation was significantly impaired by a mitogen‐activated protein kinase MEK 1/2 inhibitor, U0126 ( p < 0.05). There was no evidence of any adiponectin‐induced difference in the relative transcript abundances of AdipoR1 , AdipoR2 , AMPKα1 , AMPKα2 , PPARα and PPARγ genes. In conclusion, these results indicate that adiponectin has a positive effect on the meiotic maturation of goat oocytes through the MAPK MEK 1/2 pathway. Furthermore, the adiponectin does not affect the relative abundance of genes relevant for adiponectin signal transduction in goat oocytes.