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Effects of tumour necrosis factor‐alpha and interleukin‐1 beta on in vitro development of bovine secondary follicles
Author(s) -
Paulino Lais R. F. M.,
Cunha Ellen V.,
Barbalho Silva Anderson W.,
Souza Glaucinete B.,
Lopes Ewerton P. F.,
Donato Mariana A. M.,
Peixoto Cristina A.,
MatosBrito Bruno G.,
Hurk Robert,
Silva José Roberto V.
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13199
Subject(s) - follicular phase , andrology , tumor necrosis factor alpha , antrum , ovarian cortex , biology , cytokine , in vitro , endocrinology , medicine , chemistry , immunology , ovary , ovarian tissue , biochemistry , stomach
The objective of this study was to determine the effects of TNF ‐α and IL ‐1β on development and survival of bovine secondary follicle culture in vitro for 18 days. Secondary follicles (~0.2 mm) were isolated from ovarian cortex and individually cultured at 38.5°C, with 5% CO 2 in air, for 18 days, in TCM ‐199 + alone (cultured control) or supplemented with 10 ng/ml IL ‐1β, 10 ng/ml TNF ‐α or both TNF ‐α and IL ‐1β. The effects of these treatments on growth, follicular survival, antrum formation, viability, ultrastructure and mRNA levels for GDF ‐9, c‐ MOS , H1foo and Cyclin B1 were evaluated. The results showed that addition of TNF ‐α to culture medium increased follicular diameter and rate of antrum formation, whereas that of IL ‐1β and a mixture of IL ‐1β and TNF ‐α did not do so. Ultrastructural analysis showed that, among the tested cytokine treatments, follicles cultured in the presence of TNF ‐α had the best‐preserved oocytes and granulosa cells. The presence of TNF ‐α, IL ‐1β or both did not influence the expression of mRNA s analysed. In conclusion, in contrast to IL ‐1β, TNF ‐α promotes growth of and antrum formation in in vitro cultured bovine secondary follicles, while their ultrastructure and viability were maintained.