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Effect of cumulus cells and sperm concentration on fertilization and development of pig oocytes
Author(s) -
Li R,
Liu Y,
Pedersen HS,
Callesen H
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13184
Subject(s) - polyspermy , human fertilization , andrology , sperm , biology , embryogenesis , oocyte , anatomy , embryo , microbiology and biotechnology , medicine
Contents Effect of sperm concentrations and cumulus cells ( CC s) on porcine IVF was re‐evaluated using current improved IVM and IVC system. Our results showed that both CC s and sperm concentration had significant effect on penetration rate, frequency of polyspermy and embryonic development. The best IVF results were obtained with oocytes with CC s fertilized with 0.5 × 10 5 sperm/ml. Such an IVP system works on both sow and gilt oocytes.