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Disaccharide combinations and the expression of enolase3 and plasma membrane Ca 2+ ATP ase isoform in sturgeon sperm cryopreservation
Author(s) -
Xi MD,
Li P,
Du H,
Qiao XM,
Liu ZG,
Wei QW
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13134
Subject(s) - sperm , trehalose , cryopreservation , biology , extender , disaccharide , citrus × sinensis , cryoprotectant , sperm motility , sucrose , andrology , biochemistry , chemistry , food science , microbiology and biotechnology , botany , orange (colour) , medicine , embryo , organic chemistry , polyurethane
Contents Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane‐impermeable cryoprotectants, and enolase3 ( ENO 3) and plasma membrane Ca 2+ ATP ase isoform ( PMCA 2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus . We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm. A low concentration of sucrose plus trehalose (S 15 T 15 ) was optimal. Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L 15 T 15 ) gave the best results for both A. sinensis and A. dabryanus . Enolase3 and PMCA 2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting. Relative ENO 3 and PMCA 2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis . The results showed that relative ENO 3 and PMCA 2 expression levels were the highest at L 15 T 15 in cryopreserved A. sinensis sperm. There were significant positive correlations between ENO 3 expression and percentage membrane integrity, and between PMCA 2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight‐line velocity and average path velocity; p < .05 ) in cryopreserved A. sinensis sperm. Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO 3 and PMCA 2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis .