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Histological and immunohistochemical evaluation of granulosa cells during different stages of folliculogenesis in bovine ovaries
Author(s) -
Teh APP,
Izzati UZ,
Mori K,
Fuke N,
Hirai T,
Kitahara G,
Yamaguchi R
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13132
Subject(s) - folliculogenesis , biology , cytokeratin , anti müllerian hormone , immunohistochemistry , ovary , follicle , granulosa cell , vimentin , endocrinology , desmin , medicine , ovarian follicle , andrology , pathology , hormone , microbiology and biotechnology , embryogenesis , immunology , embryo
Contents Bovine granulosa cells ( GC ) vary in their morphological aspect during different stages of folliculogenesis. In this study, 10 morphologically normal bovine ovaries were collected to study the structural aspects of different stages of GC using intermediate filament protein antibodies including cytokeratin AE 1/ AE 3 ( AE 1/ AE 3), vimentin, nectin‐4 and desmin. Hormonal immunolocalization was assessed using the immunomarkers anti‐Müllerian hormone ( AMH ) and inhibin alpha. In addition, tumour markers and proliferation markers using c‐erbB‐2 oncoprotein and proliferating cell nuclear antigen, respectively, were investigated. The immunolabelling of AE 1/ AE 3 in GC was strongest in the early follicle stage and gradually decreased when reaching the Graafian follicle stage. Its immunolabelling increased again as the stage progressed from stage I to stage III . The immunolabelling of inhibin alpha was inversely proportional to that of AE 1/ AE 3 in the developing ovarian follicles as their immunolabelling is opposite to each other during folliculogenesis. AMH was immunopositive in almost all GC stages in different intensities and percentages, except for some negative staining in the atretic IV follicles. The atretic IV follicle is a unique type of atretic follicle that shows Call‐Exner body formation, which was mainly found in older cows in this study. The distinct patterns of immunoreactivity for various types of immunomarkers in the different GC stages will play an important role in diagnostic assistance of various follicle conditions, including cystic ovaries and GC tumours.