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Effect of local scrotal heating on the expression of tight junction‐associated molecule Occludin in boar testes
Author(s) -
Zhang Z,
Fan X,
Xi H,
Ji R,
Shen H,
Shi A,
He J
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13131
Subject(s) - occludin , tight junction , andrology , immunohistochemistry , blot , blood–testis barrier , sertoli cell , biology , medicine , endocrinology , spermatogenesis , immunology , microbiology and biotechnology , biochemistry , gene
Contents The aim of this study was to determine whether local scrotal heating (42°C, for 1 hr) had an effect on the expression of tight junction ( TJ )‐associated molecule Occludin in boar testes. Adult boars (Landrace, n = 6) were used and randomly divided into two groups ( n = 3 each). Three boars were given local scrotal exposure to 42°C for approximately 1 h with a home‐made electric blanket of controlled temperature as local scrotal heating group, the other three boars received no heat treatment and were left at standard room temperature as control group. After 6 hr, all boars were castrated and the testes were harvested. qRT ‐ PCR , Western blotting and immunohistochemistry were used to explore the expression and localization of Occludin. qRT ‐ PCR and Western blotting showed that the protein and mRNA levels of Occludin significantly decreased in local scrotal heating group as compared to the control. Furthermore, immunoreactivity staining of Occludin was localized at the sites of the blood–testis barrier ( BTB ) and formed an almost consecutive and strong immunoreactivity strand in the control, while Occludin was limited to Sertoli cells ( SC s) and no obvious immunoreactivity strand was present in local scrotal heating group. These data indicated that local scrotal heating decreased the expression of TJ ‐associated molecule Occludin, which may be involved in heat‐induced spermatogenesis damage.