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Identification of perivascular and stromal mesenchymal stem/progenitor cells in porcine endometrium
Author(s) -
Wiater J,
Niedziela M,
Posmysz A,
Wartalski K,
Gajda B,
Smorąg Z,
Rajfur Z,
Karasiński J
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13109
Subject(s) - mesenchymal stem cell , stromal cell , biology , progenitor cell , stem cell , population , endometrium , microbiology and biotechnology , side population , pathology , cancer research , cancer stem cell , endocrinology , medicine , environmental health
Contents Mammalian uterus contains a population of mesenchymal stem/progenitor cells that likely contribute to endometrial regeneration during each reproductive cycle. In human and mouse, they reside in perivascular, epithelial and stromal compartments of the endometrial functionalis and basalis. Here, we aimed to identify tissue resident cells expressing mesenchymal stem cell markers CD 29, CD 44, CD 90, CD 105, CD 140b and CD 146 in the porcine endometrium. We used single immunofluorescence and Western blotting. Each of these markers was detected in small cells surrounding endometrial blood vessels. CD 105 and CD 146 were also expressed in single stromal cells. A few stromal and perivascular cells showed the presence of pluripotency marker Oct4 in the cytoplasm, but not in the nucleus, which may imply they are not truly pluripotent. Endometrial cell cultures were examined for the expression of CD 29, CD 44, CD 90, CD 105 and CD 140b proteins and tested in wound‐healing assay and culture model of chemotaxis. In conclusion, our results demonstrate perivascular location of prospective mesenchymal stem/progenitor cells in the porcine endometrium and may suggest that stromal CD 105 + and CD 146 + cells represent more mature precursors originating from their perivascular ancestors.