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Acute stimulation of a smooth muscle constrictor by oestradiol‐17β via GPER 1 in bovine oviducts
Author(s) -
Nishie T,
Kobayashi Y,
Kimura K,
Okuda K
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13108
Subject(s) - gper , oviduct , endocrinology , medicine , receptor , biology , stimulation , chemistry , estrogen receptor , cancer , breast cancer
Contents Oviducts play roles in reproductive processes, including gametes transport, fertilization and early embryo development. Oviductal transport is controlled by various factors such as endothelins ( EDN s) and nitric oxide ( NO ), smooth muscle contracting and relaxing factor, respectively. EDN s and NO production depend on an ovarian steroid hormone, oestradiol‐17β (E2) and E2 quickly exerts their biological functions through G protein‐coupled oestrogen receptor 1 ( GPER 1), which mediates rapid intracellular signalling. Because follicular fluid which contains a high concentration of E2 enters the oviduct, we hypothesized that E2 in the follicular fluid participates via GPER 1 in producing EDN s and NO . To test this hypothesis, we investigated 1) the expression and localization of GPER 1 in bovine oviductal tissues and 2) rapid effects of E2 via GPER 1 on EDN 1, EDN 2 and inducible NO synthase ( iNOS ) expression in cultured bovine oviductal isthmic epithelial cells. GPER 1 was observed in the oviductal epithelium, stroma and smooth muscle, and its expression was highest in the isthmus. Short‐term treatments (≤1 hr) of E2 increased EDN 2 mRNA expression in the isthmic epithelial cells, although E2 did not affect EDN 1 and iNOS mRNA expressions. Results of GPER 1‐selective agonist G‐1 and GPER 1‐selective antagonist G‐15 treatments revealed acute stimulation by E2, which is mediated via GPER 1. The overall findings suggested that E2 in follicular fluid rapidly stimulates EDN 2 expression via GPER 1 in the isthmic epithelial cells. Follicular fluid may play a role in retention of the ovulated oocyte in the end of ampulla by contracting the isthmus for successful fertilization.