z-logo
Premium
Melatonin reduces apoptotic cells, SOD 2 and HSPB 1 and improves the in vitro production and quality of bovine blastocysts
Author(s) -
Marques TC,
Silva Santos EC,
Diesel TO,
Leme LO,
Martins CF,
Dode MAN,
Alves BG,
Costa FPH,
Oliveira EB,
Gambarini ML
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13097
Subject(s) - in vitro maturation , andrology , melatonin , blastocyst , chemistry , embryo culture , sod2 , in vitro , biology , cryopreservation , antioxidant , embryo , embryogenesis , endocrinology , medicine , superoxide dismutase , microbiology and biotechnology , biochemistry
Contents Effects of adding different concentrations of melatonin (10 −7 , 10 −9 and 10 −11  M) to maturation (Experiment 1; Control, IVM  + 10 −7 , IVM  + 10 −9 , IVM  + 10 −11 ) and culture media (Experiment 2; Control, IVC  + 10 −7 , IVC  + 10 −9 , IVC  + 10 −11 ) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10 −9  M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM  + 10 −9 , IVC  + 10 −9 , IVM / IVC  + 10 −9 ). In Experiment 1, maturated oocytes from Control and IVM  + 10 −9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC  + 10 −7 (43.5%; 56.7%) and IVC  + 10 −9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC  + 10 −9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC  + 10 −9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM / IVC  + 10 −9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC  + 10 −9 treatment also had a higher mRNA expression of antioxidant gene ( SOD 2) compared to the Control, as well as the heat shock protein ( HSPB 1) compared to the IVM  + 10 −9 . Reactive oxygen species production was greater in the IVM / IVC  + 10 −9 treatment group. In conclusion, the 10 −9  M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here