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DNA methylation and expression of imprinted genes are associated with the viability of different sexual cloned buffaloes
Author(s) -
Ruan Z,
Zhao X,
Qin X,
Luo C,
Liu X,
Deng Y,
Zhu P,
Li Z,
Huang B,
Shi D,
Lu F
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13093
Subject(s) - biology , xist , dna methylation , genomic imprinting , somatic cell nuclear transfer , methylation , epigenetics , gene , reprogramming , andrology , genetics , bisulfite sequencing , polymerase chain reaction , microbiology and biotechnology , gene expression , x inactivation , x chromosome , blastocyst , embryogenesis , medicine
Contents The DNA methylation of imprinted genes is an important way to regulate epigenetic reprogramming of donor cells in somatic cell nuclear transfer ( SCNT ). However, the effects of sexual distinction on the DNA methylation of imprinted genes in cloned animals have seldom been reported. In this study, we analysed the DNA methylation status of three imprinted genes ( Xist , IGF 2 and H19 ) from liveborn cloned buffaloes (L group, three female and three male), stillborn cloned buffaloes (S group, three female and three male) and natural reproduction buffaloes (N group, three female and three male), using bisulphite sequencing polymerase chain reaction ( BS ‐ PCR ). The expression levels of these imprinted genes were also investigated by quantitative real‐time PCR ( QRT ‐ PCR ). The DNA methylation levels of H19 were not significantly different among the groups. However, the Xist in female and IGF 2 in male of the S group were found to be significantly hypomethylated in comparison with the same sexual buffaloes in L group and N group ( p < .05). Furthermore, the expression levels of Xist , IGF 2 and H19 in the stillborn female cloned buffaloes of S group were significantly higher than that of the female buffaloes in the L group and N group ( p < .05). The expression levels of IGF 2 and H19 in the stillborn male cloned buffaloes in the S group were significantly higher than that of the male buffaloes in the L group and N group ( p < .05). These results indicate that Xist may be associated with the viability of female cloned buffaloes, and IGF 2 may also be related to the viability of male cloned buffaloes.