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Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture
Author(s) -
gbua T,
Guo Y,
Edman A,
Humblot P,
Morrell JM
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13069
Subject(s) - andrology , chemistry , biology , microbiology and biotechnology , medicine
Contents The purpose of this study was to investigate the effect of seminal plasma ( SP ) from bulls of known fertility on bovine endometrial epithelial cells ( bEEC ) in culture. The bEEC from passage 5, approximately 5.0–13 × 10 5 cells per flask, were challenged with SP from bulls of high or low fertility ( n = 3 and 2, respectively) or PBS (control), at 1% (75 μl) or 4% (300 μl) and were incubated for 72 hr ( n = 13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high‐ or low‐fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high‐ or low‐fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300 L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose‐dependent and fertility‐dependent.