Premium
Redox cycling induces spermptosis and necrosis in stallion spermatozoa while the hydroxyl radical (OH•) only induces spermptosis
Author(s) -
Martín Muñoz P,
AnelLópez L,
OrtizRodríguez JM,
Álvarez M,
Paz P,
Balao da Silva C,
Rodríguez Martinez H,
Gil MC,
Anel L,
Peña FJ,
Ortega Ferrusola C
Publication year - 2018
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13052
Subject(s) - oxidative stress , menadione , lipid peroxidation , andrology , sperm , chemistry , reactive oxygen species , apoptosis , oxidative phosphorylation , sperm motility , necrosis , glutathione , biochemistry , medicine , enzyme
Contents Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer of a special form of apoptosis, visible after thawing, in cryopreserved spermatozoa. To obtain further insights into the link between oxidative stress and the induction of apoptotic changes, stallion spermatozoa were induced to oxidative stress through redox cycling after exposure to 2‐methyl‐1,4‐naphthoquinone (menadione), or hydroxyl radical formation after FeSO 4 exposure. Either exposure induced significant increases ( p < 0.05) in two markers of lipid peroxidation: 8‐iso‐PGF 2α and 4‐hydroxynonenal (4‐HNE). While both treatments induced changes indicative of spermptosis (caspase‐3 activation and decreased mitochondrial membrane potential) ( p < 0.01), menadione induced sperm necrosis and a dramatic reduction in motility and thiol content in stallion spermatozoa. Thus, we provided evidence that oxidative stress underlies spermptosis, and thiol content is a key factor for stallion sperm function.