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miR‐182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro
Author(s) -
Zhang L,
Liu XR,
Liu JZ,
Song YX,
Zhou ZQ,
Cao BY
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13031
Subject(s) - in vitro , epithelium , microbiology and biotechnology , biology , genetics
Contents Proper HOXA 10 expression was essential for endometrial receptivity what was crucial for successful embryo implantation in mammalian. This study confirmed that miR‐182 regulated the expression levels of HOXA 10 by binding to its 3′ UTR , selectively downregulated HOXA 10 in goat endometrial epithelium cells ( gEEC s) but not stromal cell ( gESC s) in vitro. However, HOXA 10 and miR‐182 both up‐expressed in the goat endometrium at gestational day 15 (D15) compared with gestational day 5 (D5), suggesting that there were some other factors regulated the expression of HOXA 10 during the development of goat endometrium in vivo. What's more, HOXA 10 gene silencing ( HOXA 10‐si RNA ) resulted in gEEC s apoptosis in vitro, and it regulated the protein levels of oestrogen receptor a ( ER a), progesterone receptor B ( PR b), insulin‐like growth factor 1 receptor ( IGF 1R), BCL ‐2, pleiotrophin ( PTN ), AKT and p‐ JNK in gEEC s. Furthermore, HOXA 10 might regulate the protein levels of endometrial receptivity biomarker genes, including vascular endothelial growth factor ( VEGF ), osteopontin ( OPN ), cyclooxygenase‐2 ( COX ‐2) and prolactin receptor ( PRLR ) in gEEC s. In conclusion, miR‐182 targeted HOXA 10 selectively in EEC s in vitro, and HOXA 10 played an important role in maintaining the function of EEC s in dairy goats.