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Taurine does not improve the quality of short‐term stored rabbit spermatozoa in vitro
Author(s) -
Paál D,
Strejček F,
Tvrdá E,
Vašíček J,
Baláži A,
Chrenek P,
Massányi P
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13022
Subject(s) - taurine , andrology , acrosome reaction , acrosome , semen , incubation , sperm , propidium iodide , biology , new zealand white rabbit , in vitro , chemistry , biochemistry , medicine , apoptosis , paleontology , amino acid , programmed cell death
Contents This study examines the impact of taurine on the viability, morphology and acrosome integrity of rabbit spermatozoa in vitro. Semen samples, obtained from four to five sexually mature and healthy New Zealand White rabbits, were pooled in heterospermic semen sample. This was divided and treated with taurine in a concentration of 0 (control), 1.5, 7, 12.5, 50 mM to a final concentration of 10 8 spermatozoa/ml. The samples were then incubated at 37°C for 4 hr. A combination of fluorescent probes SYBR ‐14/propidium iodide/ PNA ‐Alexa Fluor 647 was used to assess spermatozoa viability and acrosome integrity on a flow cytometer. The sperm morphology was evaluated under a light microscope following fixation in 1.5% paraformaldehyde. The experiment was repeated three times. According to the obtained results, the spermatozoa neither could have benefit from immediate taurine treatment, nor had they after 4‐hr incubation with respect to viability and acrosome integrity. Taurine did not initially alter the total and acrosome morphology of treated spermatozoa nor has it by 4 hr upon treatment. In conclusion, taurine may have no protective effect on the viability, morphology and acrosome integrity of short‐term stored rabbit spermatozoa.