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Cellular and extracellular vesicular origins of miRNAs within the bovine ovarian follicle
Author(s) -
Andrade GM,
Meirelles FV,
Perecin F,
Silveira JC
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13021
Subject(s) - follicular fluid , biology , paracrine signalling , oocyte , microbiology and biotechnology , folliculogenesis , ovarian follicle , follicular phase , wnt signaling pathway , reproductive technology , microrna , autocrine signalling , granulosa cell , context (archaeology) , oogenesis , microvesicles , rna , rna extraction , follicle , signal transduction , endocrinology , cell culture , genetics , embryo , gene , embryogenesis , paleontology , receptor
Contents The ovarian follicle components must provide an ideal environment to ensure the success of reproductive processes, and communication between follicular cells is crucial to support proper oocyte growth. Recently, it has been demonstrated that the presence of extracellular vesicles (EVs) carrying microRNAs (miRNAs) in follicular fluid represents an important autocrine and paracrine communication mechanism inside the ovarian follicle. In this study, we tested the hypothesis that the miRNA content of EVs isolated from ovarian follicular (granulosa and cumulus–oocyte complexes) cell‐conditioned culture media is dependent upon cell type. We initially screened bovine granulosa cells ( GC s) and cumulus–oocyte complexes ( COC s), as well as their derived EV s for 348 mi RNA s using real‐time PCR , and detected 326 mi RNA s in GC s and COC s cells and 62 mi RNA s in GC s and COC s EV s. A bioinformatics analysis of the identified cell‐specific and differentially expressed mi RNA s predicted that they likely modulate important cellular processes, including signalling pathways such as the PI 3K‐Akt, MAPK and Wnt pathways. By investigating the origins of mi RNA s within the follicular fluid, the results of this study provide novel insights into follicular mi RNA content and intercellular communication that may be of invaluable use in the context of reproductive technologies, diagnostic of ovarian‐related diseases and/or the identification of biomarkers for oocyte and embryo quality.