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Cryopreservation of Nili‐Ravi buffalo ( Bubalus bubalis ) semen in AndroMed ® extender; in vitro and in vivo evaluation
Author(s) -
Ansari MS,
Rakha BA,
Akhter S
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13008
Subject(s) - extender , semen , bubalus , cryopreservation , biology , yolk , andrology , sperm , zoology , semen extender , semen quality , citric acid , sperm motility , anatomy , chemistry , biochemistry , food science , embryo , botany , genetics , medicine , ecology , organic chemistry , polyurethane
Contents The study was designed to evaluate AndroMed ® for the freezability and fertility of Nili‐Ravi buffalo semen. Semen was collected from four adult Nili‐Ravi buffalo ( Bubalus bubalis ) bulls for 3 weeks (replicate). Semen ejaculates from each buffalo bull were divided into three aliquots. One aliquot was used for evaluation of motility, plasma membrane integrity, livability, viability, DNA integrity and normal apical ridge. Remaining two aliquots were diluted (37°C; 50 × 10 6 spermatozoa/ml) in tris ‐citric egg yolk or AndroMed ® extender and cryopreserved in 0.5 ml French straws. After thawing, per cent post‐thaw motility (47.9 ± 0.8, 49.2 ± 1.7), plasma membrane integrity (44.4 ± 1.2, 46.8 ± 1.8) and normal apical ridge (81.4 ± 0.3, 83.2 ± 0.3) were recorded similar ( p > .05) in tris ‐citric egg yolk and AndroMed ® extender. Higher ( p < .05) percentage of sperm livability (70.5 ± 1.4 and 64.4 ± 1.0), viability (67.5 ± 1.5 and 61.5 ± 0.6) and DNA integrity (97.0 ± 0.3 and 93.4 ± 0.21) were recorded in AndroMed ® compared to tris‐citric egg yolk post‐thaw. Values for all the aforementioned spermatozoal quality parameters were observed lower ( p < .05) in frozen‐thawed compared to fresh semen irrespective of the experimental extenders. Fertility rates of buffalo semen did not differ ( p > .05) either cryopreserved in tris ‐citric egg yolk or AndroMed ® extender (45.5% vs. 49%). It is concluded that AndroMed ® is capable in protecting the buffalo bull sperm during freeze‐thawing process and can be adopted safely for routine use replacing the tris‐citric egg yolk extender in artificial insemination programme.