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Antioxidative effect of BHA in soya bean lecithin‐based extender containing Glycerol or DMSO on freezing capacity of goat semen
Author(s) -
Rahmatzadeh M,
Kohram H,
Zare Shahneh A,
SeifiJamadi A,
Ahmad E
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13007
Subject(s) - extender , cryoprotectant , chemistry , butylated hydroxyanisole , semen , glycerol , butylated hydroxytoluene , food science , sperm motility , cryopreservation , andrology , zoology , biochemistry , biology , antioxidant , medicine , embryo , organic chemistry , polyurethane , microbiology and biotechnology
Contents The aim of this study was to evaluate the effects of butylated hydroxyanisole (0 or 4 mM ) along with different concentrations (5 or 7%) of glycerol (G) and dimethyl sulphoxide ( DMSO ) as cryoprotectant ( CPA s) on freezability of goat semen. Semen was collected from four bucks (3–4 years) twice a week for five weeks. The pooled ejaculates were diluted with extender containing two different concentrations of G or DMSO in combination with BHA . Afterwards, the diluted samples were loaded into 0.25 ml straws and frozen using a standard protocol. After thawing motility parameters, viability, membrane integrity and total abnormality were assessed. The Results showed that the presence of BHA in extender, type and level of CPA s as main factors had significant effects on goat sperm viability, total and progressive motility after freezing–thawing processes ( p < .05). Also, the interaction of BHA (0 and 4 mM ) and levels of G or DMSO (5 or 7%) had a significant effects ( p < .05) on total motility, viability and some characteristic. In this case, the addition of 5% G or DMSO with BHA resulted in highest motility and viability than the other groups ( p < .05). The addition of G5 (with and without BHA ) increased VSL and reduced abnormality than the other groups ( p < .05). The results showed that the main effects of CPA s and CPA s level on membrane functionality were significant ( p < .05). Also there were no significance differences in the interactive effects of MDA , VCL , VAP , ALH , LIN and STR among the groups ( p > .05). Finally, it can be concluded that the use of 5% CPA s with or without BHA may result in better post‐thaw sperm quality of goat.