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The influence of delipidation on triglyceride and LIPIN 1 of porcine embryos derived from parthenogenetic activation
Author(s) -
Zeng Y,
Wang C,
Niu Y,
Chi D,
Xu M,
Si L,
Qu X,
Li J
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12987
Subject(s) - embryo , cytoplasm , lipid metabolism , microbiology and biotechnology , chemistry , cell , gene expression , oocyte , blastocyst , biology , lipid droplet , biochemistry , embryogenesis , gene
Contents Proteins in the LIPIN family play key roles in lipid synthesis mainly on triacylglycerol ( TAG ) biosynthesis, and they also act as transcriptional coactivators to regulate the expression of genes involved in lipid metabolism with other nuclear factors. Hence, this study was designed to investigate LIPIN 1 in pig oocytes and embryos by the delipidation. After delipidation, the content of lipids ( LD s) and TAG in MII oocyte was significantly reduced; however, a similar increasing tendency of TAG was shown during embryos development. Subsequently, the expression of genes related to TAG biosynthesis including GPAT 1, AGPAT 1, AGPAT 2, LIPIN 1, DGAT and the nuclear factors interacted with LIPIN 1 including PPAR α and PPAR γ was investigated. It is obvious that DGAT and GPAT 1, and LIPIN 1 increased significantly after delipidation at 1‐cell and 4‐cell stage, and the expression of PPAR α and PPAR γ also increased at 4‐cell stage. By immunofluorescence staining and Western blots, LIPIN 1 was found to exhibit a dynamic localization pattern and gradually increase with the development of delipated embryo. In the early developmental stages (1‐, 2‐ and 4‐cell stages), it was distributed over the cortical layer. But at the blastocyst stage, a homogeneous distribution of LIPIN 1 was observed in cytoplasm. At 2‐cell stage, the expression of PPAR α decreased when LIPIN 1 was interfered by small interfering RNA , but PPAR γ has no significant difference. Therefore, in this study, we find after delipidation, the content of TAG and LIPIN 1 will gradually increase during embryo development and nuclear factor PPAR α and PPAR γ can also be affected by delipidation. The interaction of LIPIN 1 and PPAR α exists in porcine embryo.