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Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep
Author(s) -
Srividya D,
Praveen Chakravarthi V,
Kona SSR,
Siva Kumar AVN,
Brahmaiah KV,
Rao VH
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12965
Subject(s) - antral follicle , folliculogenesis , in vivo , biology , insulin like growth factor binding protein , insulin like growth factor , endocrinology , medicine , ovary , in vitro , ovarian follicle , growth factor , andrology , gene expression , microbiology and biotechnology , receptor , gene , embryogenesis , embryo , genetics
Contents Expression of Kit ligand ( KL ) and insulin‐like growth factor binding protein ( IGFBP 3) genes was studied at different in vivo and corresponding in vitro stages of development of the ovarian follicles in sheep. The expression of both KL and IGFBP 3 was significantly higher in the primordial follicles relative to any other stage of development. Compared to the other stages, the KL expression in the cumulus cells from in vivo grown large antral follicles and that of IGFBP 3 in COC s’ isolated from large antral follicles matured in vitro for 24 hr were significantly higher. In the oocytes from in vivo grown ovarian follicles, the expression of KL was the same at all the stages of development. Insulin‐like growth factor binding protein 3 expression was also the same in the oocytes at all the stages of the development except for a significantly lower expression in those from antral follicles. The expression of KL in the cumulus cells decreased significantly in the in vitro grown early antral follicles but did not change further as the development progressed. The expression of IGFBP 3 in the cumulus cells from in vitro grown ovarian follicles appeared to increase as the development progressed although the increase was not significant between any two consecutive stages of development. In the oocytes in in vitro grown ovarian follicles, the expression levels of KL and IGFBP 3 genes did not change with development. It is concluded that (i) KL and IGFBP 3 genes follow specific patterns of expression during ovarian folliculogenesis and (ii) in vitro culture of preantral follicles compromises the development potential through alterations in the stage‐specific patterns of expression of these and other developmentally important genes.