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Comparison of the effects of Ham'sF10 and α MEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles
Author(s) -
Mohammadzadeh F,
Safdarian L,
Amidi F,
Mohammadzadeh A,
Mortezaee K,
Mehdinejhadiani S,
Sobhani A,
Ghasemi S,
Sargolzaei Aval F
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12945
Subject(s) - vitrification , cryopreservation , ovarian cortex , andrology , bovine serum albumin , follicular phase , chemistry , dna fragmentation , follicle , biology , endocrinology , embryo , biochemistry , medicine , fishery , apoptosis , programmed cell death
Contents The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (α MEM ) and Ham'sF10, supplemented with foetal bovine serum ( FBS ) or bovine serum albumin ( BSA ) in vitrification/warming solution on the quality and viability of sheep ovarian follicles. Vitrification method was applied for cryopreservation of sheep ovarian cortex using Ham'sF10 and α MEM supplemented with either BSA or FBS . There were five groups: Fresh, Ham'sF10+ BSA , Ham'sF10+ FBS , α MEM + BSA and α MEM + FBS . Samples were cultured for two weeks after warming. Viability and morphology of follicles and DNA fragmentation in follicles and in tissue stroma cells were analysed before vitrification/warming and following one and two weeks of culture. The Ham'sF10+ FBS and Ham'sF10+ BSA groups showed a significant decrease in follicular viability after one week of culture ( p < .05 vs. Fresh). Following two weeks of culture, all groups revealed a considerable fall in the number of viable follicles ( p < .05 vs. Fresh). There was an increase in DNA fragmentation of connective tissue cells but not in the follicles ( p < .05). Our results showed the better application of α MEM supplemented with BSA as a vitrification solution in improvement of cryopreservation effects and maintenance of follicular survival.