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Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro
Author(s) -
Ambrogi M,
Dall'Acqua PC,
RochaFrigoni NAS,
Leão BCS,
Mingoti GZ
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12923
Subject(s) - in vitro maturation , blastocyst , bovine serum albumin , cryopreservation , andrology , chemistry , embryo , in vitro , embryogenesis , cytoplasm , cysteamine , biology , biochemistry , microbiology and biotechnology , medicine
Contents We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum ( FCS ) or bovine serum albumin ( BSA )] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation ( IVM ) for 18 hr. The oocytes in the control groups were cultured (standard IVM ) for 24 hr in medium containing 10% FCS (Control FCS ) or 10% FCS and the antioxidant mixture (Control FCS +Antiox). The intracellular concentrations of reactive oxygen species ( ROS ) at the end of IVM period were lower in the oocytes subjected to simulated transport in the presence of a macromolecular supplement or the antioxidant mixture than that of the control group ( FCS : 0.62 and BSA : 0.66 vs. Control FCS : 1.00, p < .05; and Transp: 0.58 and Transp Antiox: 0.70 vs. Control FCS : 1.00, p < .05). After IVM , the mitochondrial membrane potentials of the transported oocytes were lower than those of the non‐transported oocytes ( FCS : 0.41 and BSA : 0.57 vs. Control FCS : 1.00, p < .05; and Transp: 0.48 and Transp Antiox: 0.51 vs. Control FCS : 1.00 and Control Antiox: 0.84, p < .05). The blastocyst formation rates (36.9% average) and the re‐expansion rates of vitrified‐warmed blastocysts (53%, average) were unaffected ( p > .05) by the treatments. In conclusion, supplementing the medium in which bovine oocytes are transported with antioxidants or different macromolecules did not affect their in vitro production of embryos or their cryotolerance.