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Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo ( Bubalus bubalis ) bulls
Author(s) -
Kumar P,
Saini M,
Kumar D,
Bharadwaj A,
Yadav PS
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12882
Subject(s) - malondialdehyde , biology , andrology , semen , fertility , zoology , superoxide dismutase , endocrinology , oxidative stress , medicine , anatomy , population , environmental health
Content This study was attempted to identify subfertile bulls by quantifying the endogenous levels of osteopontin ( OPN ), total antioxidant capacity ( TAC ) and malondialdehyde ( MDA ) in seminal plasma of buffalo bulls. On the basis of conception rate, buffalo bulls were classified into two groups: high‐fertile (conception rate >50%) and subfertile bulls (conception rate <40%). A total of 100 ejaculates (10 ejaculates from each bull) were collected through artificial vagina method. The concentration of OPN , TAC and catalase ( CAT ) of high‐fertile bulls was found to be higher ( p < .05) than that of subfertile bulls. Further, MDA level in seminal plasma was found to be lower ( p < .05) in high‐fertile bulls compared with subfertile bulls. The fertility status had no effect on the superoxide dismutase ( SOD ) concentration in seminal plasma of both the groups. The levels of OPN ( r = .678, p = 0.013) and TAC ( r = .648, p = .042) were found to be positively correlated with bull fertility and the level of MDA ( r = −.718, p = .019) was found to be negatively correlated with bull fertility. However, the fertility of bulls was not found to be significantly correlated with SOD , CAT and sperm motility. In conclusion, seminal OPN , TAC and MDA tended to be more realistic in identification of subfertile bulls from breeding herds.