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miRNA and piRNA expression profiles of breeder cock testes detected by next‐generation sequencing
Author(s) -
Wu SR,
Guo W,
Li YL,
Ren XC,
Lei XY,
Li XY,
Yao JH,
Yang XJ
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12880
Subject(s) - microrna , biology , gene , kegg , computational biology , piwi interacting rna , spermatogenesis , deep sequencing , gene expression , function (biology) , regulation of gene expression , genetics , genome , rna , gene ontology , bioinformatics , rna interference , endocrinology
Contents mi RNA s are small non‐coding regulatory RNA s that play key roles in diverse biological processes. In this study, we used the Solexa sequencing technique to profile mi RNA s in breeder cock testes to illustrate their functions. A total of 663 co‐expressed mi RNA s and 3,180 co‐expressed pi RNA s were detected in three libraries. Based on Mir‐X ™ mi RNA qRT ‐ PCR , three mi RNA s representing low, medium and high expression levels according to the sequencing results were selected randomly to validate the mi RNA s' expression profiles. Results suggested that the mi RNA expression profiles data could represent actual mi RNA expression levels. Moreover, target genes prediction of the co‐expressed mi RNA s and further Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed, which revealed that some candidate mi RNA s were involved in the regulation of the spermatogenesis process, spermatozoa function and testicular metabolism. In conclusion, we provided a useful resource for further elucidation of the mi RNA s' regulatory role in spermatogenesis, contributing to a preliminary database for functional and molecular mechanistic studies in testicular metabolism, spermatogenesis and other testes functions.