Premium
Antisperm antibodies in repeat‐breeding cows: Frequency, detection and validation of threshold levels employing sperm immobilization, sperm agglutination and immunoperoxidase assay
Author(s) -
Srivastava SK,
Shinde S,
Singh SK,
Mehrotra S,
Verma MR,
Singh AK,
Nandi S,
Srivastava N,
Singh SK,
Goswami TK,
Bhure SK,
Kumar H,
Ghosh SK
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12877
Subject(s) - sperm , andrology , semen , antibody , immunoperoxidase , insemination , infertility , agglutination (biology) , artificial insemination , biology , medicine , immunology , pregnancy , genetics , monoclonal antibody
Contents Antisperm antibodies have been found in repeat‐breeding( RB ) cows, and those causing agglutination and/or immobilization of sperm are considered to be closely related to unexplained infertility. However, a standard protocol for identifying antisperm antibodies ( ASA ) in cattle is not validated. Therefore, an investigation was undertaken to evaluate sperm immobilization ( SIT ), sperm agglutination ( SAT ) and immunoperoxidase ( IPT )assays for detection of ASA in serum and their respective threshold levels for confirmation. Animals (heifers, normally breeding, repeat‐breeding and pregnant animals) that were free from IBR , brucellosis and uterine infections (screened by clinical examination) were included in the study. Sperm agglutinating, sperm immobilizing and antisperm antibodies evaluated by respective assay were significantly higher ( p < .05) in RB cows compared to other groups. The SIT assay was able to identify 61% of RB caused by ASA , more than those employing SAT and IPT . Furthermore, a dilution rate of 1:5 and 1:80 (confirms 59.0 and 57.0% RB +ve)were sufficient to diagnose ASA by SAT and IPT , respectively. Results indicate the presence of __12.6% clumped spermatozoa and __ 2.6%(cut‐off value) peroxidase‐positive spermatozoa at 1:5 and 1:80 dilutions diagnosed with SAT and IPT , respectively, may be considered as repeaters arising out of ASA . Furthermore, study also showed the presence of lower incidence of ASA positivity in other groups of animals (heifer