Premium
Effect of testicular tissue lysate on developmental competence of porcine oocytes matured and fertilized in vitro
Author(s) -
Singh AK,
Naskar S,
Saikia B,
Vashi Y,
Gupta S,
Banik S,
Tamuli MK,
Pande V,
Sarma DK,
Dhara SK
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12875
Subject(s) - andrology , germinal vesicle , oocyte , in vitro maturation , biology , embryo , chemistry , anatomy , medicine , microbiology and biotechnology
Contents The objective of the present study was to investigate the effect of testicular tissue lysate ( TTL ) on developmental competence of germinal vesicle ( GV ) stage porcine oocytes. Two types of TTL were prepared through repeated freeze–thaw in liquid nitrogen, one from whole testicular tissue ( wTTL ) and other from either of four different sections of testes, namely just beneath the tunica albuginea ( TA ), from the transitional area between the seminiferous cord/tubules and the mediastinum testis ( TR ) and from the intermediate area (parenchymal tissue origin) and CE (cauda epididymis origin). The whole or section‐wise TTL treatments were given for 44 hr during in vitro maturation ( IVM ). Oocyte maturation was done in either of the two media, namely defined (high‐performance basic medium for porcine oocyte maturation, commercially available) and serum containing ( TCM 199). After maturation, oocytes were co‐incubated with fresh spermatozoa for 6 hr and then transferred to embryo culture media. Treatment of GV stage oocytes with wTTL (1 mg/ml) increased the cleavage and morula percentage rate (69.23 ± 6.23 and 48.15 ± 6.77, respectively) than that of their control (58.33 ± 8.08 and 32.54 ± 5.53, respectively) in defined media, and in serum‐containing media, cleavage and morula percentage rate were almost equal in both treatment (54.56 ± 7.79 and 34.70 ± 6.78, respectively) and control (59.52 ± 8.21 and 38.52 ± 6.54, respectively). However, effect of wTTL was not significant. In case of section‐wise TTL supplements, TR section significantly ( p < .01) improved cleavage and morula rate (58.43 ± 7.98 and 36.14 ± 6.89, respectively) followed by TA . In conclusion, present study indicates that IVM , in vitro fertilization and in vitro culture of embryo are improved in the presence of TTL , particularly its TR section. Further study is expected to reveal the principal components of TTL which may prove useful for IVM.