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Characterization of teratogenic potential and gene expression in canine and feline amniotic membrane‐derived stem cells
Author(s) -
Cardoso MT,
Pinheiro AO,
Vidane AS,
Casals JB,
Oliveira VC,
Gonçalves NJN,
Martins DS,
Ambrósio CE
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12832
Subject(s) - flow cytometry , transplantation , stem cell , mesenchymal stem cell , biology , microbiology and biotechnology , haematopoiesis , pathology , cancer research , medicine
Contents The biosafety of innovative procedures that utilize stem cells in regenerative medicine has been addressed in several studies. Previous work has showed no tumour formation following the use of feline and human amniotic membrane‐derived stem cells ( AMSC s). In contrast, tumour formation was observed when canine AMSC s were utilized. These findings suggested that feline and human, but not canine, AMSC s are suitable for cell transplantation trials. This study aimed to further evaluate the feasibility of utilizing canine AMSC s for transplantation purposes as well as for felines. We tested teratoma formation following cell injection into BALB /c nude mice and then assessed expression of haematopoietic, mesenchymal, tumorigenic, pluripotency and cellular regulation markers using flow cytometry and qPCR . The use of canine AMSC s did not result in macroscopic tumour formation as determined 60 days after transplantation. The immunophenotypic characterization by flow cytometry revealed expression of mesenchymal markers ( CD 73 and CD 90) and expression of the pluripotent marker OCT 4 and SOX 2. Quantitative PCR analysis revealed that there were no differences in the patterns of gene expression ( CD 34, CD 73, OCT 4, CD 30 and P53) between canine and feline AMSC s, with the exception of the expression of SOX 2 and CD 90.