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A three‐dimensional alginate system for in vitro culture of cumulus‐denuded feline oocytes
Author(s) -
Morselli MG,
Canziani S,
Vigo D,
Luvoni GC
Publication year - 2017
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12806
Subject(s) - oocyte , in vitro maturation , in vitro , andrology , meiosis , chemistry , biology , medicine , microbiology and biotechnology , embryo , genetics , gene
Contents In the case of high valuable individuals with very precious genetic material, widening the genetic pool including gametes with poor morphological characteristics, as cumulus‐denuded oocytes ( CDO s), could be an option. To improve the in vitro culture of low‐competence feline CDO s, an enriched three‐dimensional (3D) system in association with competent cumulus–oocyte complexes ( COC s) was developed. For this purpose, domestic cat CDO s were cultured with or without companion COC s in the 3D barium alginate microcapsules. The overall viability and the meiotic progression of feline CDO s cocultured with COC s or cultured separately in 3D or in 2D (traditional microdrops) system were compared. The 3D system was able to support viability and meiotic resumption of the feline oocytes, as well as the 2D microdrops. In 3D microcapsules, the presence of COC s resulted in a higher viability of CDO s (91.1%, p < .05), than that obtained without COC s or in 2D microdrops (71.2% and 67.3%, respectively), but the percentages of meiotic resumption were similar of those of CDO s cultured separately (55.4% vs. 40.4%, p > .05). It is notable that the presence of CDO s seemed to enhance the meiotic progression of the associated COC s. In conclusion, the 3D barium alginate microcapsules are a suitable system for feline oocytes in vitro culture, but more specific enriched conditions should be developed to improve the CDO s full competence in vitro.