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Lipid profiles of canine spermatozoa as revealed via matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Braga LT,
Bravo NRS,
Belaz KRA,
Zampieri D,
Eberlin MN,
Conforti VA
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12792
Subject(s) - sphingomyelin , mass spectrometry , semen cryopreservation , chemistry , semen , phospholipid , sperm , desorption , chromatography , matrix assisted laser desorption/ionization , andrology , biochemistry , sperm motility , cholesterol , medicine , organic chemistry , adsorption , membrane
Contents In this study, we investigated the ability of matrix‐assisted laser desorption/ionization mass spectrometry ( MALDI ‐ MS ) to characterize the lipid contents of canine spermatozoa. For that, samples of pure semen were analysed. Indeed, quite comprehensive lipid coverage was observed, and the most abundant phospholipid ions detected were from four phosphatidylcholines, that is those of m/z 760.6; 782.6; 808.6; and 830.6 and one of m/z 725.6 from a sphingomyelin. In conclusion, MALDI ‐ MS was found to offer an easy, fast, accurate, and sensitive analytical method for lipid profiling in canine spermatozoa and could be used as a tool to select sires by assessing the relationship between sperm lipid profiles and variables such as age and breeding history as well as to study the effects of cryopreservation on lipid contents.