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Reduction in the mRNA expression of sVEGFR 1 and sVEGFR 2 is associated with the selection of dominant follicle in cows
Author(s) -
Ortega Serrano PV,
Guzmán A,
Hernández–Coronado CG,
CastilloJuárez H,
RosalesTorres AM
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12777
Subject(s) - follicular phase , theca , follicle , endocrinology , angiogenesis , medicine , messenger rna , receptor , biology , vascular endothelial growth factor , theca interna , gene expression , andrology , follicular fluid , vegf receptors , oocyte , gene , microbiology and biotechnology , biochemistry , embryo
Contents The vascular endothelial growth factor ( VEGF ) is essential for follicular development by promoting follicular angiogenesis, as well as for the proliferation and survival of granulosa cells. The biological effects of VEGF are regulated by two membrane receptors, VEGFR 1 and VEGFR 2, and two soluble receptors, sVEGFR 1 and sVEGFR 2, which play an antagonistic role. Thus, the objective of this study was to identify the mRNA expression pattern of total VEGF , VEGFR 1, VEGFR 2, sVEGFR 1 and sVEGFR 2 in bovine preselected follicles ( PRF ) and post‐selected follicles ( POF ). The mRNA expression of these five genes in both granulosa cells ( GC ) and theca cells ( TC ) was compared between follicles classified as PRF and POF based on their diameter and on their ratio of estradiol/progesterone (E2/P4). Results showed a lower expression of mRNA of sVEGFR 1 and sVEGFR 2 in POF than in PRF ( p < .05). Regarding the mRNA expression of total VEGF , VEGFR 1 and VEGFR 2, there was no difference between POF and PRF follicles ( p > .05). Our results showed that the mRNA expression of VEGFR 2 and sVEGFR 1 was more abundant than the expression of VEGFR 1 and sVEGFR 2, while GC was the main source of mRNA for total VEGF . On the other hand, TC was the follicular compartment where the receptors were most expressed. Our results suggest that non‐dominant follicles maintain a greater concentration of the mRNA expression of both membrane and soluble VEGF receptors. On the other hand, follicular dominance is related to a reduction in the mRNA expression of sVEGFR 1 and sVEGFR 2, which may favour VEGF binding with VEGFR 2 and, hence, improve the follicular health and development.