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Effects of concanavalin A on the progesterone production by bovine steroidogenic luteal cells in vitro
Author(s) -
Destro FC,
Martin I,
LandimAlvarenga FDC,
Ferreira JCP,
Pate JL
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12767
Subject(s) - concanavalin a , luteal phase , in vitro , endocrinology , medicine , ovulation , chemistry , corpus luteum , andrology , biology , follicular phase , ovary , biochemistry , hormone
Contents The aim of this study was to evaluate the effects of concanavalin A ( CONA ) on the progesterone (P4) production by bovine steroidogenic luteal cells ( LC s) in vitro. Luteal cells were collected during the mid‐luteal stage (at 10–12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO 2 , with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 μg/ml); LH (100 μg/ml); CONA + LH ; LH (100 μg/ml) + prostaglandin F2α ( PGF 2α) (10 ng/ml); CONA + LH + PGF 2α. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p < .05. Culture in the presence of CONA decreased the P4‐secreting capacity of LC s on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments.