Premium
Transcriptome analysis of the uniparous and multiparous goats ovaries
Author(s) -
Wang LJ,
Sun XW,
Guo FY,
Zhao YJ,
Zhang JH,
Zhao ZQ
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12750
Subject(s) - kegg , transcriptome , biology , cashmere goat , gene , genome , genetics , database , gene expression , computer science
Contents Transcriptome analysis of Inner Mongolia Cashmere goat and Dazu black goat generated 38,772,947 and 38,771,668 clean pair end reads, respectively, which were assembled into 72,422 and 80,069 unigenes by Trinity, respectively. For Inner Mongolia Cashmere goat, 26,051 and 10,100 unigenes were assigned to gene ontology ( GO ) categories and clusters of orthologous groups, respectively. A total of 32,772 unigenes can comment to SWISS ‐Prot database, and the Kyoto Encyclopedia of Genes and Genomes Pathway database ( KEGG ) mapped 24,420 unigenes. While annotating the unigenes about Dazu black goats, we found 29,444(45.42%), 11,271 (38.28%), 36,910(56.94%) and 27,766 (42.83%) unigenes were assigned to GO database, COG database, SWISS ‐Prot database and KEGG database, respectively. In addition, we performed the bioinformatics analysis of gene expression profiling aimed at the ovarian transcriptome difference between Inner Mongolia Cashmere goat and Dazu black goat. We obtained a sequencing depth of over 5.5 million and 5.8 million tags. There were 1,133 DEG s between two species, of which 632 genes upregulated in the Dazu black goat and 501 genes downregulated compared with which in Inner Mongolia Cashmere goat. By annotating the 1,133 DEG s into KEGG database, we found 525 DEG s. And there were 68 DEG s annotated in metabolic pathways, 31 DEG s annotated in ribosome, 28 DEG s annotated in focal adhesion, 27 DEG s annotated in phagosome, 26 DEG s annotated in pathways in cancer, 25 DEG s annotated in ECM ‐receptor interaction, 23 DEG s annotated in protein digestion and absorption, 20 DEG s annotated in oxidative phosphorylation, 17 DEG s annotated in lysosome, and 16 DEG s annotated in cell adhesion molecules.