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Retracted: Reliable collection of Caspian brown trout ( Salmo trutta caspius ) sperm using a catheter
Author(s) -
Aramli MS,
Golshahi K,
Banan A,
Sotoudeh E
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12740
Subject(s) - salmo , sperm , semen , brown trout , biology , semen quality , sperm motility , zoology , semen collection , human fertilization , andrology , artificial insemination , anatomy , fishery , fish <actinopterygii> , medicine , botany , pregnancy , genetics
Contents The traditional stripping procedure for collecting fish semen is associated with the risk of urine contamination, which may significantly affect semen quality and quantity. The use of a catheter as an alternative method for semen collection may overcome this problem. Therefore, this study compared Caspian brown trout ( Salmo trutta caspius ) semen parameters (i.e. sperm density, seminal plasma osmolality, motility parameters of spermatozoa analysed using computer‐assisted sperm analysis and fertility) between the traditional stripping method and the use of a catheter. All parameter values of the semen collected with a catheter were significantly higher ( p < .05; density = 7.67 ± 1.02 × 10 9 ml −1 and osmolality = 279.28 ± 32.84 mO sm kg −1 ) than those collected with stripping method (density = 4.85 ± 0.47 × 10 9 ml −1 and osmolality = 216.42 ± 20.75 mO sm kg −1 ). Semen collected with a catheter was characterized by higher spermatozoa motility compared with sperm collected via stripping. Similarly, the fertilization ability of sperm collected with a catheter was significantly greater ( p < .05) than sperm collected with the traditional stripping method. In conclusion, collection of sperm with a catheter was shown to effectively reduce urine contamination and is therefore recommended for the collection of Caspian brown trout sperm.