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Overexpression of C/ EBP β Affects The Cell Cycle Regulators and Spermatogenesis Related Genes Expression And Function of Bovine Sertoli Cells
Author(s) -
Tang K,
Jin Y,
Chen F,
Wang L
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12724
Subject(s) - sertoli cell , spermatogenesis , biology , cyclin b1 , microbiology and biotechnology , gene , cell cycle , cyclin d1 , spermatocyte , gene expression , endocrinology , genetics , cyclin dependent kinase 1 , meiosis
Contents CCAAT /enhancer‐binding protein beta (C/ EBP β ), an important transcriptional factor, plays a pivotal role in the regulation of female germ cell development. However, the role of C/ EBP β on the development of male germ cells has not been reported. In this study, we constructed the recombinant adenovirus plasmids of bovine C/ EBP β gene and harvested the subsequent adenoviruses, and then assessed the mRNA levels of spermatogenesis‐related genes (real‐time PCR ) and secretion of inhibin B after 48 h of Ad‐C/ EBP β recombinant adenovirus infection in bovine sertoli cells ( SC s). We found that overexpression of exogenous C/ EBP β gene upregulated the mRNA expressions of spermatogenesis‐related genes, including Pdgfa , Claudin , Caspase‐3 , Occludin , kit1 and Cyclin E, and decreased the mRNA levels of Cyclin D1 . Meanwhile, overexpression of exogenous C/ EBP β gene significantly increases the amounts of secreted inhibin B. In conclusion, the results indicate that the C/ EBP β gene plays an important regulatory role in regulation of the cell cycle regulators and spermatogenesis‐related genes expression and function of bovine SCs.